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CoraLite®594-conjugated Catalase Monoclonal antibody

Catalase Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F8D7

Cat No : CL594-66765

Print datasheet

Synonyms

CAT, catalase

验证数据展示

  • FC experiment of HepG2 using CL594-66765

    FC experiment of HepG2 using CL594-66765

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Catalase (CL594-66765, Clone:1F8D7) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • FC experiment of HepG2 using CL594-66765

    FC experiment of HepG2 using CL594-66765

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Catalase (CL594-66765, Clone:1F8D7) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66765-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66765 targets Catalase in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen Catalase fusion protein Ag16916 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name catalase
Synonyms CAT, catalase
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC112219
Gene Symbol CAT
Gene ID (NCBI) 847
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04040
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.

实验方案

Product Specific Protocols
FC protocol for CL594 Catalase antibody CL594-66765Download protocol
Standard Protocols
Click here to view our Standard Protocols