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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10252

Print datasheet

Synonyms

bA276H19.3, CDC20, CDC20A, p55CDC

验证数据展示

  • IF Staining of HeLa using CL488-10252

    IF Staining of HeLa using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

  • IF Staining of U2OS using CL488-10252

    IF Staining of U2OS using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy CDC20 Ready-To-Use IHC Kit. CDC20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10252-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10252 targets Cdc20 in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen Cdc20 fusion protein Ag0295 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 20 homolog (S. cerevisiae)
Synonyms bA276H19.3, CDC20, CDC20A, p55CDC
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001088
Gene Symbol CDC20
Gene ID (NCBI) 991
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12834
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cdc20 antibody CL488-10252Download protocol
Standard Protocols
Click here to view our Standard Protocols