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Cytokeratin 8-specific Polyclonal antibody

Cytokeratin 8-specific Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27105-1-AP

Print datasheet

Synonyms

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytokeratin 8-specific, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

验证数据展示

  • WB analysis using 27105-1-AP

    WB analysis using 27105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27105-1-AP

    IHC staining of human tonsillitis using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of human tonsillitis using 27105-1-AP

    IHC staining of human tonsillitis using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CK8 antibody included
  • IF Staining of HepG2 using 27105-1-AP

    IF Staining of HepG2 using 27105-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 8-specific antibody (27105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27105-1-AP is Cytokeratin 8-specific Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Cytokeratin 8-specific fusion protein Ag25844 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 8
Synonyms CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytokeratin 8-specific, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8
Calculated Molecular Weight 54 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC008200
Gene Symbol KRT8
Gene ID (NCBI) 3856
RRIDAB_2918117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 8-specific antibody 27105-1-APDownload protocol
IHC protocol for Cytokeratin 8-specific antibody 27105-1-APDownload protocol
IF protocol for Cytokeratin 8-specific antibody 27105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Transl Lung Cancer Res

Identification of lysosomal genes associated with prognosis in lung adenocarcinoma

Authors - Houqiang Li
View Article
humanIHC

Environ Toxicol

Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer

Authors - Hua Chen
View Article