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DAD1 Polyclonal antibody

DAD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10531-1-AP

Print datasheet

Synonyms

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, DAD-1

验证数据展示

  • WB analysis using 10531-1-AP

    WB analysis using 10531-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10531-1-AP (DAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 10531-1-AP

    IP experiment of HepG2 using 10531-1-AP

    IP result of anti-DAD1 (IP:10531-1-AP, 4ug; Detection:10531-1-AP 1:700) with HepG2 cells lysate 2600 ug.

  • IHC staining of human liver cancer using 10531-1-AP

    IHC staining of human liver cancer using 10531-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10531-1-AP (DAD1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 10531-1-AP

    IHC staining of human liver cancer using 10531-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10531-1-AP (DAD1 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10531-1-AP targets DAD1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen DAD1 fusion protein Ag0814 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name defender against cell death 1
Synonyms Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, DAD-1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC007403
Gene Symbol DAD1
Gene ID (NCBI) 1603
RRIDAB_2089878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61803
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAD1 (defender against cell death 1) is a downstream target of the NFkB survival pathway and exhibits an antiapoptotic function. It is also named as OST2 (Oligosaccharyl transferase2) and catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Increased expression of DAD1 has been observed in various tumors.

实验方案

Product Specific Protocols
WB protocol for DAD1 antibody 10531-1-APDownload protocol
IHC protocol for DAD1 antibody 10531-1-APDownload protocol
IP protocol for DAD1 antibody 10531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols