DDX4,VASA Monoclonal antibody
DDX4,VASA Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1F12C3
验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, JAR cells, rat testis tissue, NCCIT cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse testis tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
67147-2-Ig targets DDX4,VASA in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | IF |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | DDX4,VASA fusion protein Ag11630 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 |
| Synonyms | DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog |
| Calculated Molecular Weight | 690aa,76 kDa; 724aa,79 kDa |
| Observed Molecular Weight | 79 kDa |
| GenBank Accession Number | BC047455 |
| Gene Symbol | DDX4 |
| Gene ID (NCBI) | 54514 |
| RRID | AB_2882446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NQI0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX4,VASA antibody 67147-2-Ig | Download protocol |
| IHC protocol for DDX4,VASA antibody 67147-2-Ig | Download protocol |
| IF protocol for DDX4,VASA antibody 67147-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability | ||
Reprod Sci Mechanism Research of DHEA Treatment Improving Diminished Ovarian Reserve by Attenuating the AMPK-SIRT1 Signaling and Mitophagy |
