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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DIABLO Polyclonal antibody

DIABLO Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10434

Print datasheet

Synonyms

DIABLO, diablo homolog (Drosophila), Diablo homolog, mitochondrial, DIABLO S, FLJ10537, FLJ25049, SMAC, SMAC3

验证数据展示

  • IF Staining of HeLa using CL488-10434

    IF Staining of HeLa using CL488-10434

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DIABLO antibody (CL488-10434) at dilution of 1:100.

  • FC experiment of HeLa using CL488-10434

    FC experiment of HeLa using CL488-10434

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DIABLO (CL488-10434) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10434-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10434 targets DIABLO in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen DIABLO fusion protein Ag0696 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diablo homolog (Drosophila)
Synonyms DIABLO, diablo homolog (Drosophila), Diablo homolog, mitochondrial, DIABLO S, FLJ10537, FLJ25049, SMAC, SMAC3
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC011909
Gene Symbol DIABLO
Gene ID (NCBI) 56616
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR28
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DIABLO antibody CL488-10434Download protocol
FC protocol for CL Plus 488 DIABLO antibody CL488-10434Download protocol
Standard Protocols
Click here to view our Standard Protocols