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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DRP1 (C-terminal) Polyclonal antibody

DRP1 (C-terminal) Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-12957

Print datasheet

Synonyms

DLP1, DNM1L, Dnm1p/Vps1p like protein, DRP1, DRP1 (C-terminal), DVLP, DYMPLE, dynamin 1 like, Dynamin 1 like protein, Dynamin like protein, Dynamin like protein 4, Dynamin like protein IV, Dynamin related protein 1, DYNIV 11, FLJ41912, HDYNIV, VPS1

验证数据展示

  • FC experiment of HeLa using CL488-12957

    FC experiment of HeLa using CL488-12957

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DRP1 (C-terminal) (CL488-12957) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12957-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12957 targets DRP1 (C-terminal) in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen DRP1 (C-terminal) fusion protein Ag3644 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynamin 1-like
Synonyms DLP1, DNM1L, Dnm1p/Vps1p like protein, DRP1, DRP1 (C-terminal), DVLP, DYMPLE, dynamin 1 like, Dynamin 1 like protein, Dynamin like protein, Dynamin like protein 4, Dynamin like protein IV, Dynamin related protein 1, DYNIV 11, FLJ41912, HDYNIV, VPS1
Calculated Molecular Weight 736 aa, 82 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC024590
Gene Symbol DNM1L
Gene ID (NCBI) 10059
RRIDAB_2919066
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00429
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DRP1 (C-terminal) antibody CL488-12957Download protocol
Standard Protocols
Click here to view our Standard Protocols