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DUSP16 Polyclonal antibody

DUSP16 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14237-1-AP

Print datasheet

Synonyms

DUSP16, KIAA1700, MAP kinase phosphatase 7, MKP 7, MKP7

验证数据展示

  • WB analysis of PC-12 using 14237-1-AP

    WB analysis of PC-12 using 14237-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human colon using 14237-1-AP

    IHC staining of human colon using 14237-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 14237-1-AP

    IHC staining of human colon using 14237-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inPC-12 cells, COLO 320 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14237-1-AP targets DUSP16 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DUSP16 fusion protein Ag5479 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual specificity phosphatase 16
Synonyms DUSP16, KIAA1700, MAP kinase phosphatase 7, MKP 7, MKP7
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC042101
Gene Symbol DUSP16
Gene ID (NCBI) 80824
RRIDAB_2878031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY84
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP16, also known as MKP-7, is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. DUSP16 dephosphorylates JUN kinases (JNKs) specifically and thereby regulates T-cell differentiation, which can be responsible for the pathogenesis of Burkitt's lymphoma and cerebral ischemia. DUSP16 was also found to induce the expression of VCAM-1 through the suppression of JNK signaling.

实验方案

Product Specific Protocols
WB protocol for DUSP16 antibody 14237-1-APDownload protocol
IHC protocol for DUSP16 antibody 14237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Discov

NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.

Authors - Ze-Qing Pu
View Article
humanWB

Int Immunopharmacol

Berberine derivative DCZ0358 induce oxidative damage by ROS-mediated JNK signaling in DLBCL cells

Authors - Qilin Feng
View Article