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验证数据展示

  • WB analysis using 67672-1-Ig

    WB analysis using 67672-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat colon using 67672-1-Ig

    IHC staining of rat colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 67672-1-Ig

    IHC staining of rat colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DVL1 Monoclonal antibody

DVL1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67672-1-Ig

产品说明书

CloneNo. 1A2A5

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

WB, IHC, ELISA

别名

Dishevelled 1, DSH homolog 1, DVL, DVL1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, ROS1728 cells, NCCIT cells, HeLa cells, MDA-MB-231 cells
Positive IHC detected inrat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67672-1-Ig targets DVL1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 DVL1 fusion protein Ag26083 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dishevelled, dsh homolog 1 (Drosophila)
别名 Dishevelled 1, DSH homolog 1, DVL, DVL1
计算分子量 75 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC050454
基因名称 DVL1
Gene ID (NCBI) 1855
RRIDAB_2882868
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14640
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.

实验方案

Product Specific Protocols
WB protocol for DVL1 antibody 67672-1-IgDownload protocol
IHC protocol for DVL1 antibody 67672-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols