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- KD/KO Validated
DYNLT1 Polyclonal antibody
DYNLT1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, human heart tissue, Sp2/0 cells |
Positive IP detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:600 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11954-1-AP targets DYNLT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | DYNLT1 fusion protein Ag2557 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dynein, light chain, Tctex-type 1 |
Synonyms | CW 1, DYNLT1, Protein CW 1, TCTEL1, tctex 1, TCTEX1 |
Calculated Molecular Weight | 113 aa, 12 kDa |
Observed Molecular Weight | 12 kDa |
GenBank Accession Number | BC029412 |
Gene Symbol | DYNLT1 |
Gene ID (NCBI) | 6993 |
RRID | AB_2230661 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P63172 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DYNLT1 antibody 11954-1-AP | Download protocol |
IHC protocol for DYNLT1 antibody 11954-1-AP | Download protocol |
IF protocol for DYNLT1 antibody 11954-1-AP | Download protocol |
IP protocol for DYNLT1 antibody 11954-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Neurosci Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells.
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Cell Res Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein. | ||
J Cell Biol Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
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Cancers (Basel) Dynein Light Chain Protein Tctex1: A Novel Prognostic Marker and Molecular Mediator in Glioblastoma. | ||
Biochem Pharmacol Cannabinoid receptor-2 expression modulates Gβ1γ2 protein interaction with the activator of G protein signalling 2/dynein light chain protein Tctex-1. | ||
J Cell Physiol Tctex-1, a novel interaction partner of Kidney Injury Molecule-1, is required for efferocytosis.
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