WB Figures
WB analysis of A431 using 66455-1-Ig (same clone as 66455-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis using 66455-1-Ig (same clone as 66455-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 66455-1-Ig (same clone as 66455-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of EC109 using 66455-1-Ig (same clone as 66455-1-PBS)
EC109 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66455-1-Ig (same clone as 66455-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 66455-1-Ig (same clone as 66455-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis using 66455-1-Ig (same clone as 66455-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 66455-1-Ig (same clone as 66455-1-PBS)
WB result of EGFR antibody (66455-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR transfected HepG2 cells. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 66455-1-Ig (same clone as 66455-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 66455-1-Ig (same clone as 66455-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
WB analysis of SKOV-3 using 66455-1-Ig (same clone as 66455-1-PBS)
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66455-1-Ig (same clone as 66455-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
FC Figures
FC experiment of A431 using 66455-1-Ig (same clone as 66455-1-PBS)
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human EGFR (66455-1-Ig, Clone:2A2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 66455-1-Ig (same clone as 66455-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EGFR (66455-1-Ig, Clone:2A2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.
