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  • Featured Product
  • KD/KO Validated

EIF2A/CDA02 Polyclonal antibody

EIF2A/CDA02 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11233-1-AP

Print datasheet

Synonyms

EIF2A, EIF2, EIF 2A, EIF, CDA02

验证数据展示

  • WB analysis of HeLa using 11233-1-AP

    WB analysis of HeLa using 11233-1-AP

    WB result of EIF2A/CDA02 antibody (11233-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HeLa cells.

  • WB analysis using 11233-1-AP

    WB analysis using 11233-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 11233-1-AP

    IP experiment of MCF-7 using 11233-1-AP

    IP result of anti-EIF2A/CDA02 (IP:11233-1-AP, 3ug; Detection:11233-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

  • IHC staining of human prostate cancer using 11233-1-AP

    IHC staining of human prostate cancer using 11233-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 11233-1-AP

    WB analysis using 11233-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11233-1-AP

    WB analysis using 11233-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 11233-1-AP

    IP experiment of MCF-7 using 11233-1-AP

    IP result of anti-EIF2A/CDA02 (IP:11233-1-AP, 4ug; Detection:11233-1-AP 1:6000) with MCF-7 cells lysate 760 ug.

  • IHC staining of human prostate cancer using 11233-1-AP

    IHC staining of human prostate cancer using 11233-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11233-1-AP

    IF Staining of HepG2 using 11233-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2A/CDA02 antibody (11233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 11233-1-AP

    FC experiment of HepG2 using 11233-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug EIF2A/CDA02 Polyclonal antibody (11233-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293T cells, HeLa cells, PC-12 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Ethacrynic acid treated HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11233-1-AP targets EIF2A/CDA02 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen EIF2A/CDA02 fusion protein Ag1753 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2A, 65kDa
Synonyms EIF2A, EIF2, EIF 2A, EIF, CDA02
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC011885
Gene Symbol EIF2A
Gene ID (NCBI) 83939
RRIDAB_2246321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

实验方案

Product Specific Protocols
WB protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IHC protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IF protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IP protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
View Article
mouseWB

Nature

Translation from unconventional 5' start sites drives tumour initiation.

Authors - Ataman Sendoel
  • KO Validated
View Article
WB

Science

Translation from the 5' untranslated region shapes the integrated stress response.

Authors - Shelley R Starck
  • KD Validated
View Article
humanWB

Science

Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.

Authors - Shelley R Starck
  • KD Validated
View Article
humanWB

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
View Article
humanWB

Sci Adv

CReP mediates selective translation initiation at the endoplasmic reticulum.

Authors - Jonathan P Kastan
View Article