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验证数据展示

  • WB analysis of HEK-293 using 66482-1-Ig

    WB analysis of HEK-293 using 66482-1-Ig

    WB result of EIF2A/CDA02 antibody (66482-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HEK-293 cells.

  • IHC staining of human prostate cancer using 66482-1-Ig

    IHC staining of human prostate cancer using 66482-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66482-1-Ig

    IF Staining of HepG2 using 66482-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF2A/CDA02 antibody (66482-1-Ig, Clone: 1G7H4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66482-1-Ig

    WB analysis using 66482-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Featured Product
  • KD/KO验证

EIF2A/CDA02 Monoclonal antibody

EIF2A/CDA02 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66482-1-Ig

产品说明书

CloneNo. 1G7H4
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EIF2A, EIF2, EIF 2A, EIF, CDA02

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66482-1-Ig targets EIF2A/CDA02 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 EIF2A/CDA02 fusion protein Ag22041 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2A, 65kDa
别名 EIF2A, EIF2, EIF 2A, EIF, CDA02
计算分子量 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC011885
基因名称 EIF2A
Gene ID (NCBI) 83939
RRIDAB_2881848
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BY44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.

实验方案

Product Specific Protocols
WB protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IHC protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IF protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pharm Biol

Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway

Authors - Yaoyao Bian
View Article
humanWB

Front Neurosci

Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease

Authors - Fahad Mubariz
View Article