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EIF3D Polyclonal antibody

EIF3D Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, yeast and More (2)

Applications

IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10219-1-AP

Print datasheet

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7



经过测试的应用

Positive WB detected inmouse brain tissue, L02 cells, A549 cells, HepG2 cells, human brain tissue, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, HepG2 cells, Ethacrynic acid treated HepG2 cells, transfected cells, N/A, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10219-1-AP targets EIF3D in WB, PLA, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, yeast samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IHC, PLA, WB
Tested Reactivity human, mouse, yeast
Cited Reactivityhuman, mouse, rat, yeast, rabbit
Immunogen EIF3D fusion protein Ag0268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit D
Synonyms eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC000328
Gene Symbol EIF3D
Gene ID (NCBI) 8664
RRIDAB_2096880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

实验方案

Product Specific Protocols
WB protocol for EIF3D antibody 10219-1-APDownload protocol
IHC protocol for EIF3D antibody 10219-1-APDownload protocol
IF protocol for EIF3D antibody 10219-1-APDownload protocol
IP protocol for EIF3D antibody 10219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

Authors - Zhen Shi
humanWB

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
humanIHC

EBioMedicine

PMID: 31669222

Authors - Hai Huang
mouseWB

Dev Cell

Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c

Authors - Kotaro Fujii
rabbitWB

EMBO J

HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.

Authors - Locker Nicolas N
humanWB

PLoS Pathog

A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.

Authors - Eoin N Leen