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EIF3D Polyclonal antibody
EIF3D Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, yeast and More (2)
Applications
IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, L02 cells, A549 cells, HepG2 cells, human brain tissue, Jurkat cells |
Positive IP detected in | A549 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U2OS cells, HepG2 cells, Ethacrynic acid treated HepG2 cells, transfected cells, N/A, MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10219-1-AP targets EIF3D in WB, PLA, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, yeast samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, IF, IHC, PLA, WB |
Tested Reactivity | human, mouse, yeast |
Cited Reactivity | human, mouse, rat, yeast, rabbit |
Immunogen | EIF3D fusion protein Ag0268 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 3, subunit D |
Synonyms | eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7 |
Calculated Molecular Weight | 66 kDa |
Observed Molecular Weight | 66 kDa |
GenBank Accession Number | BC000328 |
Gene Symbol | EIF3D |
Gene ID (NCBI) | 8664 |
RRID | AB_2096880 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O15371 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for EIF3D antibody 10219-1-AP | Download protocol |
IHC protocol for EIF3D antibody 10219-1-AP | Download protocol |
IF protocol for EIF3D antibody 10219-1-AP | Download protocol |
IP protocol for EIF3D antibody 10219-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide. | ||
Dev Cell Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c | ||
PLoS Pathog A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation. |