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  • Featured Product
  • KD/KO Validated

ENO1 Polyclonal antibody

ENO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 55237-1-AP

Print datasheet

Synonyms

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

验证数据展示

  • WB analysis of HeLa using 55237-1-AP

    WB analysis of HeLa using 55237-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 55237-1-AP

    WB analysis of HEK-293 using 55237-1-AP

    WB result of ENO1 antibody (55237-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells.

  • IHC staining of human brain using 55237-1-AP

    IHC staining of human brain using 55237-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with ENO1 antibody included
  • IHC staining of human brain using 55237-1-AP

    IHC staining of human brain using 55237-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with ENO1 antibody included
  • IP experiment of mouse brain using 55237-1-AP

    IP experiment of mouse brain using 55237-1-AP

    IP result of anti-ENO1 (IP:55237-1-AP, 4ug; Detection:55237-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IF Staining of HepG2 using 55237-1-AP

    IF Staining of HepG2 using 55237-1-AP

    Immunofluorescent analysis of HepG2 cells using 55237-1-AP (ENO1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, mouse liver tissue, HEK-293 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55237-1-AP targets ENO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enolase 1, (alpha)
Synonyms Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_001428
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
RRIDAB_10837362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross reaction with ENO2 and ENO3.

实验方案

Product Specific Protocols
WB protocol for ENO1 antibody 55237-1-APDownload protocol
IHC protocol for ENO1 antibody 55237-1-APDownload protocol
IF protocol for ENO1 antibody 55237-1-APDownload protocol
IP protocol for ENO1 antibody 55237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

EMBO Rep

Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.

Authors - Guang Yang
View Article