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ENO1 Monoclonal antibody
ENO1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human, mouse, rat, pig
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1D6E8
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-3 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67187-1-Ig targets ENO1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | ENO1 fusion protein Ag17518 种属同源性预测 |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | enolase 1, (alpha) |
| Synonyms | ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC015641 |
| Gene Symbol | ENO1 |
| Gene ID (NCBI) | 2023 |
| ENSEMBL Gene ID | ENSG00000074800 |
| RRID | AB_2882482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | P06733 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ENO1 antibody 67187-1-Ig | Download protocol |
| IF protocol for ENO1 antibody 67187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |