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ENO1 Recombinant antibody

ENO1 Recombinant Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C20

Cat No : 81478-1-RR

Print datasheet

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

验证数据展示

  • WB analysis using 81478-1-RR

    WB analysis using 81478-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 81478-1-RR

    IHC staining of mouse brain using 81478-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ENO1 antibody included
  • IF Staining of HepG2 using 81478-1-RR

    IF Staining of HepG2 using 81478-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (81478-1-RR, Clone: 2C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, pig brain tissue, HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, Jurkat cells, rat brain tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81478-1-RR targets ENO1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ENO1 fusion protein Ag1692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name enolase 1, (alpha)
Synonyms Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross-reaction with ENO2 and ENO3.

实验方案

Product Specific Protocols
WB protocol for ENO1 antibody 81478-1-RRDownload protocol
IHC protocol for ENO1 antibody 81478-1-RRDownload protocol
IF protocol for ENO1 antibody 81478-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols