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CoraLite® Plus 488-conjugated ER Polyclonal antibody
ER Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 21244-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-21244 targets ER in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) |
Tested Reactivity | human, mouse, rat |
Immunogen | ER fusion protein Ag15738 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | estrogen receptor 1 |
Synonyms | DKFZp686N23123, ER, ER alpha, Era, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1 |
Calculated Molecular Weight | 595 aa, 66 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC128573 |
Gene Symbol | ESR1 |
Gene ID (NCBI) | 2099 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P03372 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 ER antibody CL488-21244 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |