验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue |
| Positive IP detected in | human plasma tissue |
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 3 publications below |
产品信息
24295-1-AP targets Coagulation Factor II in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, plasmodium falciparum |
| Immunogen | Coagulation Factor II fusion protein Ag18385 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | coagulation factor II (prothrombin) |
| Synonyms | Prothrombin, F2, PT, EC:3.4.21.5, Activation peptide fragment 2 |
| Calculated Molecular Weight | 622 aa, 70 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC051332 |
| Gene Symbol | F2 |
| Gene ID (NCBI) | 2147 |
| RRID | AB_2879478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00734 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Coagulation Factor II antibody 24295-1-AP | Download protocol |
| IF protocol for Coagulation Factor II antibody 24295-1-AP | Download protocol |
| IP protocol for Coagulation Factor II antibody 24295-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Sci Rep Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. | ||
Sci Rep C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1. | ||
Malar J The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. | ||
PLoS Pathog Thrombin cleavage of the hepatitis E virus polyprotein at multiple conserved locations is required for genome replication |