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FAM175B Polyclonal antibody

FAM175B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26868-1-AP

Print datasheet

Synonyms

Abraxas brother protein 1, ABRO1, BRISC complex subunit Abro1, FAM175B, KIAA0157, Protein FAM175B

验证数据展示

  • IHC staining of human heart using 26868-1-AP

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 26868-1-AP

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HL-60 using 26868-1-AP

    WB analysis of HL-60 using 26868-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, HEK-293 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26868-1-AP is FAM175B Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen FAM175B fusion protein Ag22121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 175, member B
Synonyms Abraxas brother protein 1, ABRO1, BRISC complex subunit Abro1, FAM175B, KIAA0157, Protein FAM175B
Calculated Molecular Weight 415 aa, 47 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC008999
Gene Symbol FAM175B
Gene ID (NCBI) 23172
RRIDAB_2880663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15018
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM175B antibody 26868-1-APDownload protocol
IHC protocol for FAM175B antibody 26868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols