验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16747-1-AP targets Fibrinogen Beta Chain in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, plasmodium falciparum |
| 免疫原 | Fibrinogen Beta Chain fusion protein Ag10169 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibrinogen beta chain |
| 别名 | FGB, fibrinogen beta chain |
| 计算分子量 | 491 aa, 56 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC107766 |
| 基因名称 | Fibrinogen Beta Chain |
| Gene ID (NCBI) | 2244 |
| RRID | AB_11042764 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02675 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGB is the beta chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide B is cleaved from FGB by thrombin. Mutations in the gene of FGB lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Fibrinogen Beta Chain antibody 16747-1-AP | Download protocol |
| IHC protocol for Fibrinogen Beta Chain antibody 16747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Blood Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury. | ||
Biochem Biophys Res Commun Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period. | ||
Malar J The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. | ||
Brain Res Accelerated accumulation of fibrinogen peptide chains with Aβ deposition in Alzheimer's disease (AD) mice and human AD brains. |
