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验证数据展示

  • IHC staining of human pituitary using 67131-1-Ig

    IHC staining of human pituitary using 67131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 67131-1-Ig

    IHC staining of human pituitary using 67131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67131-1-Ig

    IF Staining of HepG2 using 67131-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FSHB antibody (67131-1-Ig, Clone: 1B10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

FSHB Monoclonal antibody

FSHB Monoclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 67131-1-Ig

产品说明书

CloneNo. 1B10A11

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IHC, IF/ICC, ELISA

别名

Follitropin beta chain, Follitropin subunit beta, FSH B, FSH beta, FSHB

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman pituitary tissue, human pituitary adenoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67131-1-Ig targets FSHB in IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 FSHB fusion protein Ag13788 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 follicle stimulating hormone, beta polypeptide
别名 Follitropin beta chain, Follitropin subunit beta, FSH B, FSH beta, FSHB
计算分子量 129 aa, 15 kDa
GenBank蛋白编号BC113488
基因名称 FSHB
Gene ID (NCBI) 2488
RRIDAB_2882430
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01225
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

The FSHB gene encodes the beta subunit of follicle-stimulating hormone (FSH), particularly expressed in gonadotroph cells within the anterior pituitary gland and plays a critical role in the regulation of gonadal function, pubertal maturation and reproductive processes in mammals. The transcription of the FSHB gene is critical for the production of the FSH hormone(PMID:28281143). And FSH is required for ovarian folliculogenesis in females, in males it promotes spermatogenesis in conjunction with testosterone(PMID:11739331). Mutations in FSHB gene would result in infertility both in men and women (PMID: 23766128).

实验方案

Product Specific Protocols
IHC protocol for FSHB antibody 67131-1-IgDownload protocol
IF protocol for FSHB antibody 67131-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols