Flag tag Antibody 21 Publications

Mouse Monoclonal| Catalog number: 66008-2-Ig |CloneNo.: 1E7B4

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100 μl


500 μl


1000 μl


Species specificity:
Recombinant protein, human, rat

Positive WB detected in:
Transfected HEK-293 cells, ag19172

Positive IP detected in:
Recombinant protein protein

Positive IF detected in:
Transfected HEK-293 cells

Recommended dilution:
WB : 1:1000-1:10000
IP : 0.5-4.0 ug for IP and 1:10-1:100 for WB
IF : 1:20-1:200

Product Information


Purification method:
Protein A purification


PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
Flag tag

Calculated molecular weight:

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

DDK tag, DYKDDDDK, FLAG, Flag tag

Flag tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of Flag tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the Flag tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with Flag tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This Flag-tag antibody can be used to detect the FLAGGED protein with pCMV-FLAG, p3XFLAG-CMV, pcDNA3.1-FLAG, pef-NEO-FLAG, FLAG-RIG-I, pLenti6-3XFLAG and some other vectors.

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