Flotillin 2 Monoclonal antibody
Flotillin 2 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit and More (1)
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1E11G12
验证数据展示
经过测试的应用
Positive WB detected in | pig brain tissue, A431 cells, HeLa cells, HEK-293 cells, A549 cells, NIH/3T3 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:2000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
CoIP | See 1 publications below |
产品信息
66881-1-Ig targets Flotillin 2 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, CoIP |
Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
Cited Reactivity | human |
Immunogen | Flotillin 2 fusion protein Ag28308 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | flotillin 2 |
Synonyms | ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1 |
Calculated Molecular Weight | 379 aa, 42 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC017292 |
Gene Symbol | FLOT2 |
Gene ID (NCBI) | 2319 |
RRID | AB_2882213 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14254 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Flotillin 2 antibody 66881-1-Ig | Download protocol |
IHC protocol for Flotillin 2 antibody 66881-1-Ig | Download protocol |
IF protocol for Flotillin 2 antibody 66881-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Dermatol Sci MicroRNA-486-3p promotes the proliferation and metastasis of cutaneous squamous cell carcinoma by suppressing flotillin-2. | ||
Toxins (Basel) Interaction of Clostridium perfringens Epsilon Toxin with the Plasma Membrane: The Role of Amino Acids Y42, Y43 and H162 | ||
Int J Oncol Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2 | ||
Nat Commun Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo |