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  • Featured Product
  • KD/KO Validated

G6PD Monoclonal antibody, PBS Only

G6PD Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

2A7B12

Cat No : 66373-1-PBS

Print datasheet

Synonyms

G6P, G6PD, G6PD1

验证数据展示

  • WB analysis of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    WB analysis of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    WB result of G6PD antibody (66373-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66373-1-Ig (same clone as 66373-1-PBS)

    WB analysis of HepG2 using 66373-1-Ig (same clone as 66373-1-PBS)

    Several cell lines were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G6PD antibody included
  • IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G6PD antibody included
  • IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66373-1-Ig(G6PD antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy G6PD Ready-To-Use IHC Kit. G6PD primary antibody included.

产品信息

66373-1-PBS targets G6PD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity human, mouse, rat
Immunogen G6PD fusion protein Ag21862 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glucose-6-phosphate dehydrogenase
Synonyms G6P, G6PD, G6PD1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC000337
Gene Symbol G6PD
Gene ID (NCBI) 2539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11413
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.