GAD1 Monoclonal antibody

GAD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D5B7

Cat No : 67648-1-Ig

Print datasheet

Synonyms

FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP



经过测试的应用

Positive WB detected inpig brain tissue, rat brain tissue, mouse cerebellum tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse brain tissue
Positive IHC detected inrat cerebellum tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue
Planning an IHC experiment? We recommend our IHCeasy GAD1 Ready-To-Use IHC Kit. GAD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67648-1-Ig targets GAD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse
Immunogen GAD1 fusion protein Ag21336 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glutamate decarboxylase 1 (brain, 67kDa)
Synonyms FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 67 kDa, 57 kDa
GenBank Accession NumberBC002815
Gene Symbol GAD1
Gene ID (NCBI) 2571
RRIDAB_2882848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.

实验方案

Product Specific Protocols
WB protocol for GAD1 antibody 67648-1-IgDownload protocol
IHC protocol for GAD1 antibody 67648-1-IgDownload protocol
IF protocol for GAD1 antibody 67648-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Psychiatr Res

NLRP3 in the GABAergic neuron induces cognitive impairments in a mouse model of hemorrhage shock and resuscitation

Authors - Ji-Zhen Liu
human,mouseIF,WB

Cells

CREB1 Facilitates GABAergic Neural Differentiation of Human Mesenchymal Stem Cells through BRN2 for Pain Alleviation and Locomotion Recovery after Spinal Cord Injury

Authors - Yanbing Kao