GLAST Polyclonal antibody
GLAST Polyclonal Antibody for WB,IP,IHC,IF/ICC,FC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB,IP,IHC,IF/ICC,FC,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Neuro-2a cells, C6 cells, mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Neuro-2a cells |
Positive FC detected in | Neuro-2a cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20785-1-AP targets GLAST in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB,IP,IHC,IF/ICC,FC,ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, cow |
Immunogen | GLAST fusion protein Ag14177 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | solute carrier family 1 (glial high affinity glutamate transporter), member 3 |
Synonyms | EA6, EAAT1, GLAST, GLAST 1, GLAST1, SLC1A3 |
Calculated Molecular Weight | 542 aa, 60 kDa |
Observed Molecular Weight | 50-55 kDa, 90-100 kDa |
GenBank Accession Number | BC037310 |
Gene Symbol | SLC1A3 |
Gene ID (NCBI) | 6507 |
RRID | AB_2878738 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P43003 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GLAST antibody 20785-1-AP | Download protocol |
IHC protocol for GLAST antibody 20785-1-AP | Download protocol |
IF protocol for GLAST antibody 20785-1-AP | Download protocol |
IP protocol for GLAST antibody 20785-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nucleic Acids Res H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor. | ||
Cell Rep SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice | ||
Cell Death Differ RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain. | ||
Neuropharmacology Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease. | ||
Sci Rep Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell Firing Pattern of NPC1-deficient Mice. | ||
J Pers Med Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling |