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GLI1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 61215,61216 61215

Synonyms

GLI1, Glioma-associated oncogene 1, zinc finger transcription factor, oncogene, hedgehog, TGF-b, cell cycle progression, neural stem cells, dbdabs, stem cell, stem cells, sample

验证数据展示

  • GLI1 antibody (pAb) tested by Western blot. Nuclear extract of Raji cells (20 ug) probed with GLI1 antibody (pAb) at a dilution of 1:1,000.

    GLI1 antibody (pAb) tested by Western blot. Nuclear extract of Raji cells (20 ug) probed with GLI1 antibody (pAb) at a dilution of 1:1,000.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This GLI1 antibody was raised against a peptide within the C-terminal region of human GLI1.
Full Name GLI1 antibody (pAb)
Synonyms GLI1, Glioma-associated oncogene 1, zinc finger transcription factor, oncogene, hedgehog, TGF-b, cell cycle progression, neural stem cells, dbdabs, stem cell, stem cells, sample
Molecular weight 120 kDa
GenBank accession numberNP_005260
RRIDAB_2614953
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

GLI1 (Glioma-associated oncogene 1) is a zinc finger transcription factor and oncogene. GLI1 is part of the hedgehog signaling pathway, but is also activated in response to TGF-b signaling as well. GLI1 activates transcription of a number of genes that promote cell cycle progression. GLI1 is overexpressed as a result of constitutive activation of hedgehog signaling, which plays a role in the initiation of cancer. GLI1 also promotes self-renewal of neural stem cells.