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GLUD1 Monoclonal antibody

GLUD1 Monoclonal Antibody for IF-P, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF-P, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

4G10D3

Cat No : 67026-1-Ig

Print datasheet

Synonyms

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

验证数据展示

  • WB analysis using 67026-1-Ig

    WB analysis using 67026-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67026-1-Ig

    IHC staining of human liver cancer using 67026-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

    Ready-to-use IHC kits available with GLUD1 antibody included
  • IHC staining of human liver cancer using 67026-1-Ig

    IHC staining of human liver cancer using 67026-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

    Ready-to-use IHC kits available with GLUD1 antibody included
  • IF Staining of human liver cancer using 67026-1-Ig

    IF Staining of human liver cancer using 67026-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 67026-1-Ig

    IF Staining of human liver cancer using 67026-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy GLUD1 Ready-To-Use IHC Kit. GLUD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67026-1-Ig targets GLUD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF-P, IHC, WB,ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen GLUD1 fusion protein Ag6179 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutamate dehydrogenase 1
Synonyms GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC040132
Gene Symbol GLUD1
Gene ID (NCBI) 2746
RRIDAB_2882341
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00367
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

实验方案

Product Specific Protocols
WB protocol for GLUD1 antibody 67026-1-IgDownload protocol
IHC protocol for GLUD1 antibody 67026-1-IgDownload protocol
IF protocol for GLUD1 antibody 67026-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols