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GNMT Monoclonal antibody

GNMT Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rat and More (1)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1B5E3

Cat No : 67294-1-Ig

Print datasheet

Synonyms

glycine N methyltransferase, GNMT

验证数据展示

  • WB analysis using 67294-1-Ig

    WB analysis using 67294-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67294-1-Ig

    IF Staining of A431 using 67294-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrat liver tissue, pig liver tissue, mouse liver tissue, rat pancreas tissue, mouse pancreas tissue
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67294-1-Ig targets GNMT in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity Human, Mouse, Pig, Rat
Cited Reactivityhuman
Immunogen GNMT fusion protein Ag4598 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycine N-methyltransferase
Synonyms glycine N methyltransferase, GNMT
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC032627
Gene Symbol GNMT
Gene ID (NCBI) 27232
RRIDAB_2882559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

实验方案

Product Specific Protocols
WB protocol for GNMT antibody 67294-1-IgDownload protocol
IF protocol for GNMT antibody 67294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.

Authors - Changhong Yang
View Article