CoraLite® Plus 647-conjugated GOLGA2/GM130 Polyclonal antibody
GOLGA2/GM130 Polyclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 11308-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-11308 targets GOLGA2/GM130 in IF, FC (Intra) applications and shows reactivity with human, Canine samples.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | human, Canine |
Immunogen | GOLGA2/GM130 fusion protein Ag1848 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | golgi autoantigen, golgin subfamily a, 2 |
Synonyms | Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2 |
Calculated Molecular Weight | 111 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC014188 |
Gene Symbol | GOLGA2 |
Gene ID (NCBI) | 2801 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q08379 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 GOLGA2/GM130 antibody CL647-11308 | Download protocol |
FC protocol for CL Plus 647 GOLGA2/GM130 antibody CL647-11308 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |