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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GOSR2/Membrin Monoclonal antibody

GOSR2/Membrin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7E7C7

Cat No : CL488-66134

Print datasheet

Synonyms

27 kDa Golgi SNARE protein, Bos1, GOSR2, GOSR2/Membrin, GS27, Membrin

验证数据展示

  • FC experiment of Jurkat using CL488-66134

    FC experiment of Jurkat using CL488-66134

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GOSR2/Membrin (CL488-66134, Clone:7E7C7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66134-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66134 targets GOSR2/Membrin in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, rat
Immunogen GOSR2/Membrin fusion protein Ag21393 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name golgi SNAP receptor complex member 2
Synonyms 27 kDa Golgi SNARE protein, Bos1, GOSR2, GOSR2/Membrin, GS27, Membrin
Calculated Molecular Weight 212 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC009710
Gene Symbol GOSR2
Gene ID (NCBI) 9570
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14653
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GOSR2/Membrin antibody CL488-66134Download protocol
Standard Protocols
Click here to view our Standard Protocols