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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GPR37/Pael-R Polyclonal antibody

GPR37/Pael-R Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-14820

Print datasheet

Synonyms

EDNRBL, ETBR LP 1, GPR37, hET(B)R LP, PAELR

验证数据展示

  • IF Staining of mouse brain using CL594-14820

    IF Staining of mouse brain using CL594-14820

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 GPR37/Pael-R antibody (CL594-14820) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-14820

    IF Staining of mouse brain using CL594-14820

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 GPR37/Pael-R antibody (CL594-14820) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 14820-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14820 targets GPR37/Pael-R in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P
Tested Reactivity human, mouse, rat
Immunogen GPR37/Pael-R fusion protein Ag6591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 37 (endothelin receptor type B-like)
Synonyms EDNRBL, ETBR LP 1, GPR37, hET(B)R LP, PAELR
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC040007
Gene Symbol GPR37
Gene ID (NCBI) 2861
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15354
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).

实验方案

Product Specific Protocols
IF protocol for CL594 GPR37/Pael-R antibody CL594-14820Download protocol
Standard Protocols
Click here to view our Standard Protocols