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GRIPAP1 Polyclonal antibody

GRIPAP1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15806-1-AP

Print datasheet

Synonyms

GRASP 1, GRIP1 associated protein 1, GRIPAP1, KIAA1167

验证数据展示

  • WB analysis using 15806-1-AP

    WB analysis using 15806-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15806-1-AP (GRIPAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15806-1-AP

    IHC staining of mouse brain using 15806-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15806-1-AP (GRIPAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 15806-1-AP

    IF Staining of SH-SY5Y using 15806-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GRIPAP1 antibody (15806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15806-1-AP targets GRIPAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GRIPAP1 fusion protein Ag8540 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GRIP1 associated protein 1
Synonyms GRASP 1, GRIP1 associated protein 1, GRIPAP1, KIAA1167
Calculated Molecular Weight 841 aa, 96 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC001522
Gene Symbol GRIPAP1
Gene ID (NCBI) 56850
RRIDAB_2112787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4V328
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRIP-associated protein-1 (GRASP-1), also named as GRIPAP1, is a neuron-specific effector of the small GTPase Rab4 and key component of AMPA receptor recycling machinery in dendrites. GRASP-1 is essential for maintenance of spine morphology and important for LTP. GRASP-1 connects Rab4 and Rab11 recycling endosomal domains through the interaction with target (t)-SNARE syntaxin. The MW of this protein is 97 kDa, and Catalog#15806-1-AP specially recognises the 97 kDa protein.

实验方案

Product Specific Protocols
WB protocol for GRIPAP1 antibody 15806-1-APDownload protocol
IHC protocol for GRIPAP1 antibody 15806-1-APDownload protocol
IF protocol for GRIPAP1 antibody 15806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.

Authors - Hani Goodarzi
View Article