GSTK1 Polyclonal antibody

GSTK1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14535-1-AP

Print datasheet

Synonyms

DsbA-L, GST, GST 13 13, GST class kappa, GST13, GST13 13, GSTK1, GSTK1 1, hGSTK1



经过测试的应用

Positive WB detected inHepG2 cells, HUVEC cells, Raji cells, L02 cells, mouse testis tissue, rat testis tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GSTK1 Ready-To-Use IHC Kit. GSTK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14535-1-AP targets GSTK1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsCoIP, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen GSTK1 fusion protein Ag6036 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione S-transferase kappa 1
Synonyms DsbA-L, GST, GST 13 13, GST class kappa, GST13, GST13 13, GSTK1, GSTK1 1, hGSTK1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC050715
Gene Symbol GSTK1
Gene ID (NCBI) 373156
RRIDAB_2115910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Q3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

实验方案

Product Specific Protocols
WB protocol for GSTK1 antibody 14535-1-APDownload protocol
IHC protocol for GSTK1 antibody 14535-1-APDownload protocol
IP protocol for GSTK1 antibody 14535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Proteome Res

Proteomic Analysis of Hearts from Akita Mice Suggests Increases in Soluble Epoxide Hydrolase and Antioxidative Programming are Key Changes in Early Stages of Diabetic Cardiomyopathy.

Authors - Dewey Shannamar S
mouseWB

J Gerontol A Biol Sci Med Sci

PMID: 24285747

Authors - Rojanathammanee Lalida L
mouseWB

Age (Dordr)

PMID: 25001375

Authors - Lalida Rojanathammanee
humanWB

Exp Mol Pathol

Aberrant expression of redox regulatory proteins in patients with concomitant primary Sclerosing cholangitis/inflammatory bowel disease.

Authors - Dennis R Petersen
humanCoIP

FASEB J

The role of chemerin in the regulation of cGAS-STING pathway in gestational diabetes mellitus placenta

Authors - Xuan Zhou
human,ratWB

Sci Total Environ

m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance

Authors - Jingyuan Zhang