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GSTK1 Recombinant antibody

GSTK1 Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3J1

Cat No : 81527-1-RR

Print datasheet

Synonyms

GST, GST 13 13, GST class kappa, GST13, GST13 13, GSTK1, GSTK1 1, hGSTK1

验证数据展示

  • WB analysis using 81527-1-RR

    WB analysis using 81527-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 81527-1-RR

    IHC staining of mouse testis using 81527-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSTK1 antibody included
  • IF Staining of HeLa using 81527-1-RR

    IF Staining of HeLa using 81527-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, L02 cells, Raji cells, Daudi cells
Positive IHC detected inmouse testis tissue, mouse liver tissue, human pancreas cancer tissue, human lung cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GSTK1 Ready-To-Use IHC Kit. GSTK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81527-1-RR targets GSTK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen GSTK1 fusion protein Ag6036 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutathione S-transferase kappa 1
Synonyms GST, GST 13 13, GST class kappa, GST13, GST13 13, GSTK1, GSTK1 1, hGSTK1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC050715
Gene Symbol GSTK1
Gene ID (NCBI) 373156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2Q3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity and hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

实验方案

Product Specific Protocols
WB protocol for GSTK1 antibody 81527-1-RRDownload protocol
IHC protocol for GSTK1 antibody 81527-1-RRDownload protocol
IF protocol for GSTK1 antibody 81527-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols