• Featured Product
  • KD/KO Validated

GSTP1 Polyclonal antibody

GSTP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15902-1-AP

Print datasheet

Synonyms

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI



经过测试的应用

Positive WB detected inCOLO 320 cells, human brain tissue, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, PC-3 cells, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skin tissue, human skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GSTP1 Ready-To-Use IHC Kit. GSTP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15902-1-AP targets GSTP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen GSTP1 fusion protein Ag8731 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione S-transferase pi 1
Synonyms DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI
Calculated Molecular Weight 210 aa, 23 kDa
Observed Molecular Weight 23-28 kDa
GenBank Accession NumberBC010915
Gene Symbol GSTP1
Gene ID (NCBI) 2950
RRIDAB_2116216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09211
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

实验方案

Product Specific Protocols
WB protocol for GSTP1 antibody 15902-1-APDownload protocol
IHC protocol for GSTP1 antibody 15902-1-APDownload protocol
IF protocol for GSTP1 antibody 15902-1-APDownload protocol
IP protocol for GSTP1 antibody 15902-1-APDownload protocol
FC protocol for GSTP1 antibody 15902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Mol Cell

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation

Authors - Wenxin Zhang
  • KD Validated
mouseWB

Redox Biol

GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux

Authors - Xiaoxiao Ji
  • KD Validated
mouseIF

J Cell Biol

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Authors - Wan Yun Ho
mouseWB

Part Fibre Toxicol

Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.

Authors - Ming Xu
mouseWB

Cell Death Dis

Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.

Authors - Cheng Wang
humanIHC

Oncotarget

Prognostic significance of GSTP1 in patients with triple negative breast cancer.

Authors - Guanglei Chen