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  • Featured Product
  • KD/KO Validated

GSTT2B Polyclonal antibody

GSTT2B Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17622-1-AP

Print datasheet

Synonyms

GST class theta 2, GSTT2, GSTT2B, GSTT2P

验证数据展示

  • WB analysis using 17622-1-AP

    WB analysis using 17622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17622-1-AP (GSTT2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17622-1-AP

    IF Staining of HepG2 using 17622-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GSTT2B antibody (17622-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse lung tissue, human liver tissue, human brain tissue
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GSTT2B Ready-To-Use IHC Kit. GSTT2B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17622-1-AP targets GSTT2B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GSTT2B fusion protein Ag11828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione S-transferase theta 2B (gene/pseudogene)
Synonyms GST class theta 2, GSTT2, GSTT2B, GSTT2P
Calculated Molecular Weight 244 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC071700
Gene Symbol GSTT2B
Gene ID (NCBI) 653689
RRIDAB_2116359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0CG30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GSTT2B antibody 17622-1-APDownload protocol
IF protocol for GSTT2B antibody 17622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Gerontol A Biol Sci Med Sci

PMID: 24285747

Authors - Rojanathammanee Lalida L
View Article
mouseWB

Age (Dordr)

PMID: 25001375

Authors - Lalida Rojanathammanee
View Article
mouseIHC

Sci Signal

Glutathione S-transferases promote proinflammatory astrocyte-microglia communication during brain inflammation.

Authors - Shin-Ichi Kano
  • KD Validated
View Article