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GTPBP3 Polyclonal antibody

GTPBP3 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10764-1-AP

Print datasheet

Synonyms

GTP binding protein 3, GTPBG3, GTPBP3, MSS1, MTGP1, THDF1

验证数据展示

  • WB analysis of Jurkat using 10764-1-AP

    WB analysis of Jurkat using 10764-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10764-1-AP (GTPBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10764-1-AP

    IHC staining of human breast cancer using 10764-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10764-1-AP (GTPBP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10764-1-AP

    IHC staining of human breast cancer using 10764-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10764-1-AP (GTPBP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human breast cancer using 10764-1-AP

    IF Staining of human breast cancer using 10764-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10764-1-AP (GTPBP3 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10764-1-AP targets GTPBP3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen GTPBP3 fusion protein Ag1198 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTP binding protein 3 (mitochondrial)
Synonyms GTP binding protein 3, GTPBG3, GTPBP3, MSS1, MTGP1, THDF1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC019261
Gene Symbol GTPBP3
Gene ID (NCBI) 84705
RRIDAB_2115389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969Y2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP3 antibody 10764-1-APDownload protocol
IHC protocol for GTPBP3 antibody 10764-1-APDownload protocol
IF protocol for GTPBP3 antibody 10764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo
View Article