• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human tonsillitis using 27894-1-AP

    IHC staining of human tonsillitis using 27894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27894-1-AP

    IHC staining of human tonsillitis using 27894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27894-1-AP

    IHC staining of human tonsillitis using 27894-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27894-1-AP (GZMK antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NK-92 using 27894-1-AP

    IF Staining of NK-92 using 27894-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using Granzyme K antibody (27894-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Granzyme K Polyclonal antibody

Granzyme K Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 27894-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA and More (1)

Synonyms

GZMK, Granzyme-3, Granzyme3, Granzyme 3, Fragmentin-3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 900 ug/ml

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNK-92 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

27894-1-AP targets Granzyme K in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Granzyme K fusion protein Ag27281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name granzyme K (granzyme 3; NK tryptase II)
Synonyms GZMK, Granzyme-3, Granzyme3, Granzyme 3, Fragmentin-3
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC035802
Gene Symbol GZMK
Gene ID (NCBI) 3003
RRIDAB_2881008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49863
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.

实验方案

Product Specific Protocols
IHC protocol for Granzyme K antibody 27894-1-APDownload protocol
IF protocol for Granzyme K antibody 27894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

iScience

CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response

Authors - Maolin Mu
View Article