验证数据展示
经过测试的应用
Positive WB detected in | human plasma tissue, U2OS cells, A549 cells, human plasma, HT-29 cells |
Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66280-1-Ig targets Gelsolin in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Gelsolin fusion protein Ag17916 种属同源性预测 |
Host / Isotype | Mouse / IgA |
Class | Monoclonal |
Type | Antibody |
Full Name | gelsolin (amyloidosis, Finnish type) |
Synonyms | Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN |
Calculated Molecular Weight | 782 aa, 86 kDa |
Observed Molecular Weight | 80-83 kDa |
GenBank Accession Number | BC026033 |
Gene Symbol | Gelsolin |
Gene ID (NCBI) | 2934 |
RRID | AB_2881663 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Thiophilic affinity chromatograph |
UNIPROT ID | P06396 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.
实验方案
Product Specific Protocols | |
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WB protocol for Gelsolin antibody 66280-1-Ig | Download protocol |
IHC protocol for Gelsolin antibody 66280-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |