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验证数据展示

  • WB analysis of U2OS using 66280-1-Ig

    WB analysis of U2OS using 66280-1-Ig

    U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • WB analysis of human plasma using 66280-1-Ig

    WB analysis of human plasma using 66280-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66280-1-Ig

    IHC staining of human colon using 66280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 66280-1-Ig

    IHC staining of human colon using 66280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO Validated

Gelsolin Monoclonal antibody

Gelsolin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66280-1-Ig
CloneNo. 3C4A2

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, U2OS cells, A549 cells, human plasma, HT-29 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66280-1-Ig targets Gelsolin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Gelsolin fusion protein Ag17916 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name gelsolin (amyloidosis, Finnish type)
Synonyms Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN
Calculated Molecular Weight 782 aa, 86 kDa
Observed Molecular Weight 80-83 kDa
GenBank Accession NumberBC026033
Gene Symbol Gelsolin
Gene ID (NCBI) 2934
RRIDAB_2881663
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP06396
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.

实验方案

Product Specific Protocols
WB protocol for Gelsolin antibody 66280-1-IgDownload protocol
IHC protocol for Gelsolin antibody 66280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols