HA Tag Monoclonal antibody

HA Tag Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (6)

Applications

FC, IF, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat No : 66006-2-Ig

Print datasheet

Synonyms

HA Tag, YPYDVPDYA



经过测试的应用

Positive WB detected inTransfected HEK-293 cells, HEK-293 cells, Ag19172
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells
Positive FC detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66006-2-Ig targets HA Tag in WB, PLA, IP, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications FC, IF, IP, WB, ELISA
Cited Applications ChIP, CoIP, ELISA, IF, IP, PLA, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, yeast, pig, canine
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name HA Tag
Synonyms HA Tag, YPYDVPDYA
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

实验方案

Product Specific Protocols
WB protocol for HA Tag antibody 66006-2-IgDownload protocol
IF protocol for HA Tag antibody 66006-2-IgDownload protocol
IP protocol for HA Tag antibody 66006-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell

SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9

Authors - Nora Schmidt

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
humanIP

Nat Commun

Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis

Authors - Zhikai Wang
IP

J Clin Invest

FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway

Authors - Yunjiang Zhou
WB

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang
WB

Acta Pharm Sin B

Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.

Authors - Xiliang Du