• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®647-conjugated HA Tag Recombinant antibody

HA Tag Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

IF/ICC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

6I21

Cat No : CL647-81290

Print datasheet

Synonyms

HA

验证数据展示

  • IF Staining of Transfected HEK-293 using CL647-81290

    IF Staining of Transfected HEK-293 using CL647-81290

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®647 HA Tag antibody (CL647-81290, Clone: 6I21 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inTransfected HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 81290-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-81290 targets HA Tag in IF applications and shows reactivity with recombinant protein samples.

Tested Applications IF/ICC
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HA Tag
Synonyms HA
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

实验方案

Product Specific Protocols
IF protocol for CL647 HA Tag antibody CL647-81290Download protocol
Standard Protocols
Click here to view our Standard Protocols