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HIF-1 alpha Monoclonal antibody, PBS Only

HIF-1 alpha Monoclonal Antibody for WB,Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H3C12

Cat No : 66730-1-PBS

Print datasheet

Synonyms

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF 1α, HIF1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF1α, Member of PAS protein 1, MOP1, PASD8

验证数据展示

  • WB analysis of HeLa using 66730-1-Ig (same clone as 66730-1-PBS)

    WB analysis of HeLa using 66730-1-Ig (same clone as 66730-1-PBS)

    Untreated and cobalt chloride treated HeLa and HepG2 cells were subjected to SDS-PAGE followed by western blot with 66730-1-Ig (HIF1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with YY1 antibody as loading control. This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

Samples need to be treated with hypoxia stimulation.

产品信息

66730-1-PBS targets HIF-1 alpha in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA
Tested Reactivity Human
Immunogen HIF-1 alpha fusion protein Ag15198 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF 1α, HIF1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF1α, Member of PAS protein 1, MOP1, PASD8
Calculated Molecular Weight 826 aa, 93 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC012527
Gene Symbol HIF1A
Gene ID (NCBI) 3091
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16665
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .