CoraLite® Plus 488-conjugated Histone H3 Monoclonal antibody
Histone H3 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, chicken, zebrafish, wheat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A2A3
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68345-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68345 targets Histone H3 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, chicken, zebrafish, wheat samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | Human, mouse, rat, chicken, zebrafish, wheat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | histone cluster 1, H3a |
| Synonyms | H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Histone H3 antibody CL488-68345 | Download protocol |
| FC protocol for CL Plus 488 Histone H3 antibody CL488-68345 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
