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CoraLite® Plus 488-conjugated HuR/ELAVL1 Monoclonal antibody

HuR/ELAVL1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11H2

Cat No : CL488-66549

Print datasheet

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

验证数据展示

  • IF Staining of L02 using CL488-66549

    IF Staining of L02 using CL488-66549

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite® Plus 488-conjugated HuR/ELAVL1 antibody (CL488-66549, Clone: 1A11H2 ) at dilution of 1:100, CL594-Phalloidin (red).

  • IF Staining of HepG2 using CL488-66549

    IF Staining of HepG2 using CL488-66549

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HuR antibody (CL488-66549, Clone: 1A11H2 ) at dilution of 1:800.

经过测试的应用

Positive IF detected inL02 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66549-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66549 targets HuR/ELAVL1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen HuR/ELAVL1 fusion protein Ag2249 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Synonyms ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC003376
Gene Symbol ELAVL1
Gene ID (NCBI) 1994
RRIDAB_2883354
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15717
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HuR/ELAVL1 antibody CL488-66549Download protocol
Standard Protocols
Click here to view our Standard Protocols