Human CD14 ELISA Kit0 Publications

Catalog number: KE00104



96 T


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Product name:
Human CD14 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

2.9 pg/mL

125-8000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatants 95% 75%-122%
Human plasma 109% 91%-127%

Samplenmean (pg/mL)SDCV%
1 20 313.1 8.1 2.6
2 20 1,254.3 60.9 4.9
3 20 4,597.7 217.6 4.7

Samplenmean (pg/mL)SDCV%
1 24 293.8 14.0 4.8
2 24 1,187.0 51.1 4.3
3 24 4,156.8 258.3 6.2

Product overview:
KE00104 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD14 ELISA kit is to be used to detect and quantify protein levels of endogenous CD14. The assay recognizes human CD14. A monoclonal antibody specific for CD14 has been pre-coated onto the microwells. The CD14 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for CD14 is added to detect the captured CD14 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

CD14, CD14 molecule

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS. Soluble forms of CD14 (sCD14) have been detected in cell culture supernatants and in human serum and urine, either shedded from the cell surface or released from intracellular pools. Increased release of sCD14 from monocytes is observed after stimulation with various agents, and sCD14 may therefore be a marker for activation of monocytes/macrophages.

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