Human CXCL10 ELISA Kit0 Publications

Catalog number: KE00128

规格

单价

96 T

¥2600

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Overview


Product name:
Human CXCL10 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Saliva

Assay type:
Sandwich

Sensitivity:
13.4 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human plasma 94% 83%-103%
Human saliva 99% 89%-112%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1093.1 66.9 6.1
2 20 433.3 17.1 3.9
3 20 124.5 9.0 7.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1055.3 94.1 8.9
2 24 454.3 22.5 5.0
3 24 127.7 9.6 7.5

Product overview:
KE00128 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL10 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL10. The assay recognizes human CXCL10. A monoclonal antibody specific for CXCL10 has been pre-coated onto the microwells. The CXCL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for CXCL10 is added to detect the captured CXCL10 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
C X C motif chemokine 10, C7, crg 2, CXCL10, Gamma IP10, gIP 10, IFI10, INP10, IP 10, mob 1, SCYB10, Small inducible cytokine B10
Background

CXCL10 ( also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.


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