Human G-CSF ELISA Kit0 Publications

Catalog number: KE00106

规格

单价

96 T

¥2600

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Overview


Product name:
Human G-CSF ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.4 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Cell culture supernatants 98% 91%-108%
Human plasma 103% 88%-117%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 433.8 11.6 2.7
2 20 106.5 4.0 4.6
3 20 24.8 4.7 5.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 416.7 24.9 6.0
2 24 104.2 5.0 4.8
3 24 24.7 0.9 3.7

Product overview:
KE00106 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The G-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous G-CSF. The assay recognizes human G-CSF. A monoclonal antibody specific for G-CSF has been pre-coated onto the microwells. The G-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for G-CSF is added to detect the captured G-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin
Background

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.


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