Human GDF15 ELISA Kit0 Publications

Catalog number: KE00108

规格

单价

96 T

¥2600

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Overview


Product name:
Human GDF15 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Plasma, Serum, Cell culture supernatants, Urine

Assay type:
Sandwich

Sensitivity:
0.3 ng/mL

Range:
1.25-40 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
serum 83% 73%-91%
plasma 79% 71%-92%
Cell culture supernatants 107% 93%-119%
Urine 85% 73%-99%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 6.4 0.6 9.4
2 20 15.1 1.6 10.4
3 20 40.6 2.9 7.1

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 6.8 0.4 5.1
2 24 13.9 0.9 6.3
3 24 42.5 1.4 3.3

Product overview:
KE00108 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GDF-15 ELISA kit is to be used to detect and quantify protein levels of endogenous GDF-15. The assay recognizes human GDF-15. A polyclonal antibody specific for GDF-15 has been pre-coated onto the microwells. The GDF-15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for GDF-15 is added to detect the captured GDF-15 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB
Background

Growth differentiation factor 15 (GDF-15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily. GDF-15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. Its levels are elevated in diseases such as acute respiratory distress syndrome, pulmonary hypertension and heart failure. GDF-15 is associated with cancers, cardiovascular diseases, and inflammatory diseases. GDF-15 may have a paracrine role in the modulation of adipose tissue function and body fat mass. GDF-15 is highly expressed in the placenta. GDF-15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy.


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